IBRA's Apicultural Abstracts

Readers' Guide to IBRA's Apicultural Abstracts

638.178.B; 615.5
1066/91 KEDZIA, B.; IWASZKIEWICZ, J.; GEPPERT, B.
[Pharmacological investigations on ethanolic extract of propolis.]
Badania farmakologiczne etanolowego wyciagu z propolisu. Herba Polonica (1988) 34 (4) 243-253 [Pl, en, Ba] Inst. Roslin 
Przetworów Ziearskich, ul. Libelta 27, 61-707 Poznan, Poland.
	Laboratory animals (rats, mice) were injected with concentrated ethanolic extract of propolis, at a 
dose of l00-500 mg/kg. The following pharmacological effects were observed: reduction of blood pressure, 
sedative activity, protection of liver tissue against carbon tetrachloride, protection against stomach ulcer 
formation and maintenance of serum glucose. Also, the extract given at a dose of 1-5 mg/cm³, increased the 
smooth muscle tension of a rabbit's isolated ileum. The extract showed neither diuretic, nor anti-bleeding, nor 
anti sclerotic activity.
[Author.]


638.178.B; 593.1
1067/91 HOLLANDS, I.; MIYARES, C.; PIMIENTA, R.
[Quality control of Propolisina (alcoholic extract of propolis) used as a coccidiostat, by means of a 
biological method.]
Control de la calidad de la Propolisina (extracto alcohólico de propòleos) utilizado como coccidiostático mediante un método 
biológico. Revista Cubana de Ciencias Veterinarias (1988) 19 (4) 319-326 [Es, cn, fr, ru, Bb) Fac. Biología, Univ. La Havana, 
Cuba.  		-
Alcoholic extracts of propolis have been shown to act against coccidia (parasitic protozoa), but the 
quality of these extracts as coccidiostats is variable, as has been shown in trials on infected rabbits using 
material originating from hives in different localities. The present paper describes a technique for using a free-
living aquatic protozoan, Chilomonas paramecium, for screening propolis preparations in the laboratory. 
Similar results were obtained to those of the test in rabbits, and it was concluded that for a propolis 
preparation to be classed as a good coccidiostat, it must achieve 100% mortality of C. paramecium in 8 ml of 
water within 10 minutes of 6 drops of the extract being added.

638.178.B&615; 616.23A
1068/91 SCHELLER, S.~ ALEKSANDROWICZ, J.; NIKODEMOWICZ, Ê.; CZUBA, Z. P.; KRÒL, W.; ZYDOWICZ, G.; 
MALINOWSKA, B.: KIELOCH-SZKODA, M.
[Trials of immunoregulation in patients with chronic bronchitis.]
Próby immunoregulacji w przewlektym zapaleniu oskrzeli. Immunolgia Polska (1989) 14 (3 /4) 304-305 [Pl, Bc] Dept. 
Microbiology, School of Medicine, 41-808 Zabrze-Rokitnica, Poland.
A total of 260 steel workers suffering from bronchitis were treated for 24 days by various methods 
including local and systemic regulation of the immune system and local treatment with an ethanolic extract of 
propolis (EEP) in a physiological salt solution. The best results were obtained in patients treated with EEP 
inhalations, together with propolis tablets and applications of dolomite.
[Author.]

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638.178.B; 616.988.7; 547.26-11
335/94 SERKEDJIEVA, J.; MANOLOVA, N.; BANKOVA, V.
[Anti-influenza Virus effect of some propolis constituents and their analogues (esters of substituted 
cinnamic acids).]
 Journal of Natural Products (1992) 55 (3) 294-297 [En, Bc ] Institute of Microbiology, Bulgarian Academy of Sciences, Acad. 
Georgy Bonchev Str., bl. 26, 1113 Sofia, Bulgaria.
	Six esters of substituted cinnamic acids were tested; these synthetic compounds were identical with 
or similar to compounds present in the ethyl ether fraction of propolis. The anti viral effects of the compounds 
were studied in in vitro tests with 4 influenza viruses. Isopentyl ferulate significantly inhibited (to the same 
degree as the whole ethyl ether fraction) the infectious activity of one influenza virus (A/Hong Kong (H3N2)), 
and also the production of haemagglutinins in embryonated hen'; eggs.
[P. Walker]
 
638.178.8&612.017.3; 612.112.A
638,178.B; 612.017.3; 576.8,097.28; 582.623.28; 547.972.2
668/94 HAUSEN, B. M.; EVERS, P.; STUWE, H.-T.; KÖNIG, W. A.; WOLLENWEBER, E.
[Propolis allergy (IV). Studies with further sensitizers from propolis and constituents common to 
propolis, poplar buds and balsam of Peru.]
Contact Dermatitis (1992) 26 (1) 34-44 [En, Bb] Department of Dermatology, University Hospital, Martinistrasse 52, 2000 Hamburg 
20, Germany.
Of 26 compounds investigated for their sensitising capacity in guinea pigs, 19 were common to propolis 
and poplar bud exudates and 14 were also found in balsam of Peru. Four caffeates, including 3-methyl-2-
butenyl caffeate and benzyl isoferulate, were strung sensitizers. Patch tests in propolis-sensitive patients 
revealed 3-methyl-2-butenyl caffeate and phenyl methyl caffeate as the major sensitizers. Benzyl isoferulate 
produced positive results in 1 Of 3 patients on whom it was tested.
[J. M. Gedye]


638.178.B
669/94 NOWOTTNICK, K.
[Propolis: production, use, recipes.] 
Propolis: gewinnung - anwendung - rezepte. Graz, Austria; Leopold Stocker Verlag (1993) 100 pp. ISBN 37020-0653-2 [De, Bd, 
Price sch 178, DM 25]
	The introductory sections or this book include information on the history of propolis, the collection 
of propolis by bees and its composition. Part 3 describes several methods for producing propolis. Part 4 
discusses Its use in the treatment of various disorders and Part 5 gives formulas. The book is illustrated with 
clear colour photographs and line drawings, and there is a bibliography of 57 items.
[P. Walker]


638.178.B; 576.8.097.28; 547.56
671/94 WOLLENWEBER, E.~ HAUSEN, B. M.~ GREENAWAY, W.
[Phenolic constituents and sensitising properties of propolis, poplar balsam and balsam of Peru.] 
Bulletin de Liaison - Groupe Polyphenols (1990) 15, 112-120 [En, Bc] Institut für Botanik der Technischcn Hochschule, 
Schniitspahnsirasse 3, 6100 Darmstadt, Germany.
Published results of some recent studies on the composition of propolis and poplar bud exudate are 
reviewed. It is concluded that the lipophilic bud exudate is the major, if not the sole source, of propolis. 
Variations in propolis composition, and thus in Its chemical and anti microbial properties, are pointed out. It 
is reported that about 10% of people using propolis preparations are predisposed to sensitisation, and propolis 
can cause contact dermatitis. The major allergens in propolis are pentenyl caffeates The possibility of 
increased therapeutic use of poplar balsam (from buds) and of the rather similar balsam of Peru (a secretion of 
the tree Myroxylon balsamum v, pereira) is discussed. Balsam of Peru does not have the major or minor 
allergens present in propolis, but it contains esters of benzoic acid and cinnamic acid, which may cause 
allergic reactions.
[P. Walker]

638.178.A; 638.178.B; 547.581.2
107684 MATSUKA, M. 
[Content of benzoic acid 111 royal jelly and propolis.]
Honeybee Science (1993) 14 (2) 79-80 [Ja, en, Bj] Inst. Honeybee Sci., Tamagawa Univ., Machida-shi, Tokyo 194, Japan.
	Benzoic acid contents were 8-13 mg/kg in royal jelly and 3.3 g/kg in propolis.

638.178.B; 543.544
1078/94 ACKERMANN, T.
[Fast chromatographic study of propolis crudes.]
Food Chemistry (1991) 42 (2) 135-138 [En, Ba] Hagilboa Street 18, Jerusalem 94314, Israel.
	Rapid thin-layer chromatography was used to separate some of the compounds in propolis. 
Differences were found in the TLC patterns of crude propolis samples from different geographical sources. 
Propolis extracts showed the same patterns as their starting materials except that a minor band was absent 
from extract patterns. This method might be useful for the separation of compounds from propolis so that they 
can be identified.
[P. Walker]
 
638.178.B; 615.779; 576.858
1079/94 KALETA, E. F. 
[Effect of honey bee (Apis mellifera) propolis 011 the growth of small- and large-plaque variants of 
pigeon herpes virus in vitro.] 
Einfluss von Propolis der Honigbiene (Apis mellifera L.) auf die Vermehrung einer Klein- und Gross-Plaque-Variante des Tauben-
Herpesvirus in vitro. Tierärztliche Umschau (1991) 46 (9) 553-556 [De, Bc] Institut fiir Geflugelkrarlkheiten der Justus Liebig, 
Universität Giessen, Frankfurter Strasse 87, 6300 Giessen, Germany.
	None of the different propolis formulations tested had a direct disinfectant-type effect on the herpes 
virus. However, all the formulations partly restricted growth of the virus in a cell 'culture. Five' components of 
propolis (caffeic acid, luteolin, guercetin, fisetin and 3-O-caffeoyl-quinic acid) each had a cleat concentration-
dependent effect of restricting growth of the virus.
[P. Walker]

638.178.B; 615.9
1080/94 CUETO, D. J. DEL
[Propolis is not toxic] 
	Los propòleos no son tòxicos.
Colmenar (1993) No, 1, 49 [Es, Bj]

638.178.B; 547.26; 615.9
1081/94 COLMENAR
[Alarm in Argentina.]
Alarma en la Argentina. Colmenar (1993) No. 1, 48 [Es, Bj]
	Ingestion of medicinal preparations of propolis adulterated with a highly toxic alcohol, used for 
industrial purposes, caused the deaths of 23 people.


638.178.B(043); 615.779; 576.858
1082/94 SAUVAGER, F.
[Contribution to the study of anti viral properties of propoIis.]
Contribution à l'étude des propriétés antivirales de la propolis. Thèse Doctorat, Université de Rennes I, France (1992) 292 pp. [Fr, 
Bd] Faculté de Pharmacie, Université de Rennes 1, 35043 Rennes Cedex, France.
	The antiviral activity of propolis was studied using adenovirus type 2, herpes virus (HSV-1, HSV-1-
R, HSV-2), stomatitis virus and polio virus. Propolis exerted its effects both intracellularly and against the 
viral envelope. Studies of ethyl ether extracts of propolis containing a number of flavonoids, phenolic acids 
and a caffeic acid derivative showed that these compounds in various combinations had a synergistic effect.
[Author]

638.178.B(043); 547.56; 543.872
1083/94 FICART, F.
[Attempts to characterise propolis.]
Essais de caractérisation de la propolis. Mémoire Ingénieur Industriel, Institut Supérieur Industriel, Huy», Belgium (1991) 214 pp. 
[Fr, Bdo] Institut Supérieur Industriel, Laboratoire de Biotechnologie, Rue Saint-Victor 3, 4500 Huy, Belgium.
	This review, with 138 references, covers the following aspects of propolis : consideration of theories 
concerning its origin; histological, botanical and geographical origin; collection and use by honey bees; 
harvesting by the beekeeper; the market for propolis; physicochemical properties; Standardisation. Propolis 
specimens from various areas of Wallone (southern part of Belgium) and from China, Poland and Mauritius 
were analysed in order to determine qualitatively and quantitatively the presence of phenolic compounds in the 
aromatic fraction of propolis obtained by ethanol extraction. Fourteen benzoic and coumaric acid derivatives, 
2 benzaldehyde derivatives and coumarin were detected by GC and TLC. Three flavonoids (chrysin, quercetin, 
galanguin) were also detected, by TLC only. Anti oxidative properties were investigated by a kinetic method 
(determination of' the mean oxidation index of dry propolis following Vakhonina's process) and a 
potentiometric method, but these methods gave no Satisfactory results.
[Author]
 
638.178.B
1432/94 MARTINEZ D'AMATO, J. C.
[Use of plastic mesh for the production and harvesting of propolis.]
Empleo de mallas plásticas para produccion y cosecha dc propoleos. Apicultura Moderna (1993 ) No. 6, 8-13 [Es, Bj] LIDAP Soc. 
Com., Dr. Juan J. de Amézaga 2014, Montevideo, Uruguay.
	Details are given of different types of plastic mesh which can be placed over the frames in a hive for 
the bees to propolise. Trials in 1990, in Uruguay and Argentina, are reported in which the average weight of 
propolis obtained per mesh varied from 35 g to 163.14 g in areas of different vegetational types.
[D. G. Lowe)

638.178.B; 599.323.4A; 615.9
1433/94 KANEEDA, J.; NISHINA, T.
[Safety of propolis. Acute toxicity.]
Honeybee Science (1994) 15 (1) 29-33 [Ja, en, Bj] Api Co. Ltd., l -1 Kanou-Sakurada-machi, Giju 500, Japan.
	Propolis samples from Brazil and China were investigated. Suspensions prepared
from ethanol extracts of propolis were fed to 5-wk-old mice, at doses of 2.23,4.0 g/kg

638,178.B; 599.323.48; 616.33
1434/94 GIRAL, T.; ACHONG, M.; RUIZ, M.; FERNÁNDEZ, S.
[Study of the protective effect of propolis in gastric lesion models induced by 96% ethanol and 0.lM 
HCI in rats.] 
	Estudió del efecto protector del propóleo con los modelos de Índucción de lesiones gástricas con etanol 96% y HCI 0,1M 
en ratas. Ciencia y Técnica en la Agriculltura, Apicultura (1990) 6, 61-69 [Es, en, Bj ] Estaciòn Experimental Apicola, Carratera El 
Cano - Wajay, km 0, Arroyo Arenas, La Lisa, Ciudad de La habana, Cuba.

638.178.B; 599.32~.4A; 591.]-056; 576.8.097.3
344/95 DIMOV' V.; ÌVANOVSKA, N.; BANKOVA, V,; POPOV, S,
[Immunomodulatory action of propolis: IV. Prophylactic activity against Gram-negative infections 
and adjuvant effect of the water-soluble derivative.]
Vaccine (1992) 10 (12) 817-8?3 [En, Bc] Department of Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, 26 
G. Bonchev St, Sofia 1113. Bulgaria.
	A wax-free, water-soluble derivative of propolis was found to increase the resistance of mice to 
experimental infection with Gram-negative bacteria. In in vitro tests, however, it did not inhibit the growth of 
some strains. It is suggested that the propolis derivative had an effect on the animal's defence system. The 
antigenic properties of the derivative were also examined. These and other results suggest that the capacity of 
the propolis derivative to prevent infections may be largely due to macrophage activation.
[P. Walker]

638.178.B; 547.972.2; 545.84AX
345/95 BANKOVA, V. ; CHRISTOV, R. ; STOEV, G.; POPOV, S.
[Determination of phenolics from propolis by capillary gas chromatography.]
Journal of Chromatphotography, ('992) 607 (1) 150-153 [En, Bb] Institute of Organic Chemistry with Centre of Phytochemistry, 
Bulgarian Academy of Sciences, 1 1 13 Sofia, Bulgaria.
A procedure using capillary gas chromatography with an internal standard has been developed for the 
determination of the main biologically active phenolics of propolis; the flavonoid aglycones pinocembrin and 
galangin, and caffeic acid and its ß-phenyl-ethyl ester.
[Author]

638.178.B; 547.56; 545.84AX
346/95 CHRISTOV, R.; BANKOVA, V.
[Gas chromatographic analysis of underivatized phenolic constituents from propolis using an electron-
capture detector.]
Journal  of Chromatography, (1992) 623 (1) 18?-185 [En, Bb] Institute of' Organic Chemistry with Centre of Phytochemistry, 
Bulgarian Academy of Sciences, Acad. G. Bonchev Str. bl. 9. 113 Sofia, Bulgaria.
Underivatized phenolic constituents from propolis (flavonoid aglycones, phenolic acids and their esters) 
were analysed by capillary gas chromatography using an electron capture detector. The analysis was possible 
because or the good electron-capture response of' these compounds, which belong to the so-called conjugated 
electrophoresis.
[Author]
 
638,178.B; 615.779.B; 615.779; 576.851.252.A
347/95 KROL, W.; SCHELLER, S. ; SHANI, J.; Ì'IETSZ, G.; CZUBA, Z.
[Synergistic effect of ethanolic extract of propolis and antibiotics on the growth of Staphylococcus 
aureus.]
Arzneimittel Forschung (1993) 43-1 (5) 607-609 [En, de, Bc] Department of Microbiology and Immunology, Silesian School of 
Medicine, Zabrze-Rokitnica, Poland.
	Ethanolic extract or propolis (EEP), known to possess marked antibacterial activity, was incubated 
with 8 common antibiotics in culture medium containing a fixed amount of a standard strain of 
Staphfylococcus aureus. The antibiotic compounds used were: penicillin G, doxycycline, streptomycin, 
cloxacillin, chloramphenicol, ceffradine, ampicillin and polymyxin B. They were used in varying levels, 
ranging between 0.000005 and 125.0 µg/ml or units, resp. Firstly, their minimal inhibitory concentrations 
were established in the absence of EEP, then EEP was added in concentrations up to 6110 #g/ml. EEP had a 
marked synergistic effect on the antibacterial activity of streptomycin and cloxacillin, and a moderate 
synergistic effect on all the others, except ampicillin.
[Author)

638.178.B; 547.972.2; 595.782.Galleria; 591.342.A
348/95 JOHNSON, K. S.; EISCHEN, F. A.; GIANNASI, D. E.
[Chemical composition Of north American bee propolis and biological activity towards larvae of 
greater ,wax moth (Lepidoptera: Pyralidae).]
Journal of Chemical Ecology (1994) 20 (7) 1783-1791 [En, 25 ref.) Department of Botany, University or Georgia, Athens, GA 
30602, USA.
Bee propolis is a sticky amalgamation of plant resins collected by Apis mellifera and is used for filling cracks 
and repairing combs in hives. Propolis contains a diversity or compounds of plant origin and is reported to 
have medicinal, anti microbial, insecticidal and phytotoxic properties. The physical and chemical composition 
of North American samples of propolis from several sites in North America was determined and tested for 
activity against larvae of Galleria mellonella, a common apiary pest. The amount of methanol-extractable resin 
in samples from Ohio and Georgia ranged from 24 to 79% by weight. Propolis collected from hives in Ohio 
were more chemically diverse (over 30 compounds detected by paper chromatography), than material from 
south Georgia (fewer than 10 major compounds), and contained a lower proportion of methanol-insoluble
beeswax. The paper chromatographic surveys revealed little variation in the chemical profile of specific hives 
over a 6 month period, and no differences between propolis from adjacent hives. Four flavanoids were 
identified from propolis collected in Ohio: kaempferol, galangin, 3,3'-di-methoxyquercetin and 3-
methoxykaempferol. When mixed into artificial diet, fractionated propolis reduced larval growth of G, 
mellonella, but not dramatically. An array of phenolics reported from propolis (caffeic acid, chrysin, ferulic 
acid, galangin, kaempferol and quercetin) were bioassayed individually for effects on larvae but none reduced 
larval growth at the concn tested, suggesting that G, mellonella are intolerant of some phenolics in their diet.

638.178.B; 543.872; 547.587.52
349/95 YAMAUCHI, R.; KATO, K.; OIDA, S. ; KANAEDA, J.; UENO, Y.
[Benzyl caffeate, an anti oxidative compound isolated from propolis.]
Bioscience, Biotechnology and Biochemistry, (1992) 56 (8) 1321-1322 [En. Bc] Department of Food Science, Faculty of' 
Agriculture, Gifu University, Gifu 501-11, Japan.
	Propolis was obtained from Japan, China, Brazil and USA, and extracted with 60% methanol. The 
anti oxidative activity of the balsam obtained was measured by its inhibition or methyl linoleate autoxidation. 
The balsam from a sample of Chinese propolis was the most anti oxidative, and the main component 
responsible for this property was identified as benzyl caffeate. HPLC showed that the amounts of benzyl 
caffeate in the propolis balsams varied from nil (not detected) to 160 mg/g.
[D. G. Lowe]
 


638.178.B; 576.858; 615.779.B
350/95 TATEFUJI, T.; YAMAUCHI, H.; IKEDA, M.; ANDO, S.; KURIMOTO, M.
[Effect of Brazilian propolis on infectivity of viruses.]
Shoyakugaku Zasshi = Japanese Journal of Pharmacognosy (1993) 47 (1 ) 60-64 [Ja, en, Bb] Fujisaki Institute. Hayashibara 
Biochemical Laboratories, 675-1 Fujisaki, Okayama 702, Japan.
	The effect of a partially purified 85% ethanol extract of Brazilian propolis on the infectivity or VSV 
(vesicular stomatitis virus), HVJ (hemagglutinating virus of' Japan), SV-40 (simian virus 40) and HSV-1 
(herpes simplex virus type-1), was studied. The virus infectivity was evaluated in vitro by plaque formation in 
cultured cells. The propolis extract reduced virus infectivity directly only when the propolis extract was added 
to the cells after incubation of viruses. The plaque reduction ratios by 10 µg/ml propolis extract were 28.8%, 
76.2%, 61,1% and 100%. respectively,, for VSV. HVJ. SV-40 and HSV-1. [Author]

638.178.B; 593.161.13
755/95 HIGASHI, K. O.; CASTRO, S. L. DE
[Propolis extracts are effective against Trypanosoma cruzi and have an impact on its interaction with 
host cells.]
 Journal of Ethnopharmacology (1994) 43 (2) 149-155 [En. 30 ref. ] (Departamento de Ultra-estruturae Biologia Celular) Instituto 
Oswaldo Cruz, Fundaçào Oswaldo Cruz, Av. Brazil 4365, CEP 21045-900 Rio de Janeiro RJ, Brazil.
	Propolis, a natural resin produced by honey bees, that displays strong anti microbial activity, has 
been used as a chemotherapeutic agent ;since ancient times. The anti-proto-zoan properties of different 
propolis; extracts were ;studied regarding Trypanosoma cruzi and its interaction with host cell;. Ethanolic 
(EEP) and dimethylsulfoxide extracts (DEP) were both active against the 3 forms of the parasite, with the 
former being more active than the latter against amastigotes and trypomastigotes. Total analysis of  
bloodstream trypomastigotes was observed after 24 h in the presence of EEP at a concentration of 100 jig/of. 
The effect was found to be temperature dependant. Treatment of' infected peritoneal macrophages and heart 
muscle cells with EEP strongly inhibited infection levels. The utilisation of propolis as a possible 
antitrypanosomal agent is discussed.

638.178.B; 619; 616.-001.4; 6i 6.-1J01.17
756/95 SUMANO LOPEZ, H.; CAMBERROS, L. O.; OCAMPO. A. A. DE
[Comparative evaluation of a mixture of propolis and Aloe vera with commercial wound healing
products.]
Evaluación comparativa de la mezcla propoleo zabila con cicatrizantes comerciales. Vetrinaria Mexico (1989) 20 (4) 407-414 [Es, 
en, Bc] Departamento de Fisiología y Farmacología, Facultad de Medicina Veterinaria y Zootecnia, Universidad
Nacional Autónoma de México, 4510 México.
	The healing properties of a mixture of propolis essential oil (PEO) and Aloe vera gel (AVG ) (1:10) 
were compared to those of PEO and AVG alone, and to several established wound and bum healing agents. In 
trial A, PEO, AVG, and PEO/AVG mixture were tested in rats on first and second intention wound healing, 
and on second and third degree
burns; in trial B, the efficacy of the same substances was compared with commercial wound healing products 
in bacterially contaminated wounds in rats; in trial C, the PEO/AVG mixture was used in 56 clinical cases of 
dogs suffering from clean, moderately contaminated, or severely contaminated ;surgical wounds. In all 3 trials, 
the superior healing properties of the PEO/AVG mixture were apparent in terms of better histological 
appearance, superior wound strength and reduced bacterial contamination. The PEO/AVG mixture remained 
stable during the 10-day study period; the efficacy and low cost of this mixture make it highly recommendable 
for use in veterinary medicine.
[Author] 

638.178.B
l126/95 MARGHITAS, L.; SABÀU, A. L.; CORNOIU, I.; MUDURE, E.
[Research on optimising the technology of obtaining propolis.]
Cercetàri privind optimizarea tehnologiei de obtinere a propolisului. Buletinul Institutului Agronomic Cluj-Napoca. Seria
Zootehnica si Medicifici Veterinara (1992) 46, 21-24 [Ro, en, Ba] Universitatea de Stiinte Agricole, Str. Mànàstur nr. 3, 3400 Cluj-
Napoca, Romania.
Plastic mesh nets placed on top of the frames gave the best yields of propolis.
 
638.178.B
l127/95 KEDZIA, B.; HOLDERNA-KEDZIA, E. 
[Chemical composition of propolis in up-to-date studies.]
Sklad chemiczyny propolisu w swietle dotychczasowych badan Herba Polonica (1991) 38 (2) 95-l10 [Pl, en, Ba] Instytut Roslin 1 
Przetworów Zielarskich, ul. Libelta 27, 61-707 Poznan, Poland.
	This review, with 90 references, summarises recent findings on the composition of propolis. The 
largest groups of propolis compounds are the phenolic derivatives (77), flavonoids (47), trace elements (30) 
and terpenes (22).
[Author] 

638.178.B; 543.872; 665.3
l128/95 HEMEIDA, H. H.; ABD-ALFATTAH, M. A.
[1- The anti microbial and antioxidant activity of propolis as a natural honeybee product. 
2- The antioxidant efficiency on cotton seed oil.]
Bulletin of Faculty of Agriculture, University of Cairo (1993) 44 (3 ) 649-662 [En, ar, Bc] Food Science & Technology Dept., 
Faculty of Agric., Cairo Univ., Cairo, Egypt.
	Five propolis samples, collected from hives in 3 areas of Egypt, were extracted with 96% ethanol. 
The extracts were added to cottonseed oil at 200 ppm to test their antioxidant effect. The oven stability was 2-
8 times better than that of untreated control samples of oil. The propolis extracts also retarded development of 
rancidity and increase in thiobarbituric acid value. Results for the different propolis extracts varied quite 
widely; values for oil samples treated with butylated hydroxyanisole (the recommended synthetic additive) 
were intermediate.
[P. Walker]

638,178.B; 615.779.B
l129/95 ABD-AL-FATAH, M. A.; HEMEIDA, H. H.; NOUR, M. E. 
[The anti microbial and antioxidant activity of propolis as a natural honeybee product: 
1. The anti microbial activity of propolis.]
 Bulletin of Faculty of Agriculture, University of Cairo (1993 )
44 (3) 637-648 [En, ar, Bc] Dept. of Economic Entomology & Pesticides, Fac. of Agric.,
Cairo Univ., Cairo, Egypt.
	Propolis was collected from Apis mellifera colonies in 3 areas of Egypt. The anti microbial 
properties of  ethanol extracts of propolis (50-250 ppm) were assayed on 11 species of micro-organisms. The 
growth of the fungus Aspergillus niger was not affected at lower concentrations the yeast Candida albicans 
was more sensitive than a species of Saccharomyces. Among the bacteria tested, the most sensitive were 
Staphylococcus aureus and S. thermophilus. Spore-forming species (Bacillus subtilis and 2 others) and the 
Gram-negative Escherichia coli were very sensitive to the propolis extracts. The result showed some 
differences between samples from different areas.
[P. Walker]

638.178.B; 615.779.B; 616.2
1130/95 FOCHT, J.; HANSEN, S. H. ; NIELSEN, J. V.; BERG-SEGERS, A. VAN DEN; RIEZLER,
R.
[Bactericidal effect of propolis in vitro against agents causing upper respiratory tract infections.]
Arzneimittel Forschung (1993 ) 43-II (8) 921-923 [En, de, Bc] Institute
for Laboratory Medicine, Zum Schürmannsgraben 30, D-47441 Moers, Germany.
	The antibacterial activity of a water extract of propolis (13%) was determined in in vitro tests with 5 
Gram-negative, 7 Gram-positive and 4 anaerobic strains of bacteria 1;<>laicd from patients with upper 
respiratory tract infections. At concentrations of 6.25 - l 00 g propolis extract/litre, all bacteria were killed. 
However, such concentrations can be achieved only by local application not by systemic administration.
[P. Walker]
 


638.178.B; 543.872; 546.21
l131/95 PASCUAL, C.; GONZALEZ, R.; TORRICELLA, R. G.
[Scavenging action of propolis extract against oxygen radicals.]
Journal of Ethnopharmacology (1994) 41 (1/2) 9-13 [En, 17 ref.] National Center for Scientific Research, Clinical Biochemistry
Department, PO Bo>t 6990, Cubanacan, Playa, La Habana, Cuba.
	The ethanolic extracts of 2 types of' Cuban propolis (R and P) showed a similar manner of 
scavenging action against different species of oxygen radicals which were generated by specific chemical 
reactions. Chemiluminescence produced by super oxide generated from the xanthine-xanthine oxidase 
reaction was 50% inhibited by approximately 5 µg/ml of propolis R and 9.5 µg/ml of propolis P and by 
catechin (0.15 µg/ml ) and super oxide dismutase (72 ng/ml). Alkoxy radical scavenging effect was similar to 
that produced by 0.11 µg/ml of *-tocopherol: inhibition of chemiluminescence by 50% was
caused by approximately 0.6 µg/ml of both propolis preparations. The results indicate that the anti oxidative 
properties of both propolis samples could be attributed to their free radical scavenging activity against alkoxy 
radicals and to a lesser degree against super oxide.

638.178.B; 615.779.B
l132/95 KUJUMGIEV, A.; BANKOVA, V.; IGNATOVA, A.; POPOV, S.
[Antibacterial activity of propolis, some of its components and their analogs.]
Pharmazie (1993) 48
(10) 785-786 [En, 9 ref.] Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.

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638,178.B; 599.323.48; 616.-002.2
343/96 KHAYYAL, M. T.; EL-GHALAZY, M. A.; EL-KHATIB, A. S.
[Mechanisms involved in the anti inflammatory effect of propolis extract.]
Drugs under Experimental and Clinical Research (1993) 19 (5) 197-203 [En] Dept. Pharmacol., Fac. Pharm., Kasrel-Aini Str.; Cairo 
University, Cairo, Egypt.
	Propolis extract contains amino acids, flavonoids, terpenes and cinnamic acid derivatives. In various 
in vitro models propolis extract was shown to inhibit platelet aggregation and to inhibit eicosanoid, suggesting 
that it might have potent anti inflammatory properties. A 13% aqueous extract was tested orally in 3 dose 
levels (1, 5 and 10 m1/kg) on the carrageenin rat paw oedema model and on adjuvant-induced arthritis in rats. 
In both models, the extract showed potent dose-related anti inflammatory activity, which compared well with 
that of diclofenac (as a reference standard). The extract was then tested on an isolated sensitised guinea pig 
lung to study its effect on the release of  prostaglandins, leucotrienes and histamine, it is concluded that 
propolis extract has potent anti inflammatory properties in vivo.  Its activity can be well  correlated with its 
effects on the release of various mediators of inflammation.

638.178.B
342/96 SHAMRO, L. P. 
[Propolis collection in the commodity apiary.]
Bdzhil'nitstvo (1994) 21, 79-81 [Uk, m, Bj] Poltavskii Filial Institutu BdzhiInitstva im P. l. Prokolovicha UAAN, Kiev, Ukraine.

638.178.B; 547.56
344/96 TYKHONOV, O. I.; YARNYKH, T, H.; VYSHNEVSKAYA, L, I.
[Investigation of the physicochemical and technological properties of a hydrophilic phenol propolis 
preparation.]
 Farmatsevtichnii Zhurnal (Kiev) (1992) No. 5/6, 53-56 [Uk, m, en] Kharkov Pharm. Inst., Kharkov, Ukraine.
	A hydrophilic phenolic preparation from propolis was soluble in water and hardly soluble in 95% 
ethanol, practically insoluble in ether, chloroform, acetone, and other organic solvents. Some of its properties; 
were evaluated: moisture absorption, volume density, friability, extrusion power. The composition of phenol 
compounds in the preparation was analysed by one- and two-dimensional chromatography. The complete UV 
spectrum of the preparation was evaluated; the highest optical density was observed at wavelengths from 220 
to 390 nm. The absorption maximum was at 29t) nm.
 
638.178.B; 615.779.B
345/96 ABDULSALAM, K. S.; MOHAMED, M. Ì.; EL-NAWAWY, M. A.
[Effect Of Propolis on some bacterial species.]
Egyptian Journal of Phytopathology (1989) 21 (1) 61-68 [En, arj] College Agric. Food. Sci., King Faisal Univ., Al-Hassa 31982, 
Saudi Arabia.
	A sample of propolis was collected from honey bee (Apis mellifera) colonies housed in Langstroth 
hives at Al-Hassa, Saudi Arabia. A propolis-ethanol mixture (1:15) was prepared. The unfractionated extract 
(PEE) was then used to find out its anti microbial activities on 13 bacterial species (5 Gram-positive, 8 Gram-
negative). Eight extract levels, 50-2000 ppm, were incorporated into dextrose-yeast extract medium. All Gram-
positive species were inhibited by 100 ppm PEE in the medium (bacillus cereus, ß. subtilis, Staphylococcus 
aureus, S. epidermis and Streptococcus pyogenes).  Of the Gram - negative species, Enterobacter cloacae, and 
Proteus vulgaris were inhibited at 400 ppm, and Pseudomonas aeruginosa, Serratia marcescens, and Serratia 
sp. at 800 ppm. The other 3 species (Escherichia coli, Klebsiella pneumoniae and Salmonella typhimurium) 
were inhibited at 1200 ppm of PEE. it 1; concluded that PEE could he used as an antibacterial agent against 
Gram-positive species.

638.178.R; 612.017.3
346/96 EICHENSEER, M.; EISELE, S.; DISCH, R.
[Allergic contact dermatitis to propolis and Unguentum leniens.]
Gleichzeitiges Vorliegen von Kontaktallergien auf Propolis und Unguentum leniens. Allergologie (1994) 17 (6) 265-267 [De, cn, 4 
ref.] Tobelmühlestrasse 2, CfI-7270 Davos-Platz, Switzerland.
	The cases of 2 patients aged 20 and 41 years with allergic contact dermatitis to propolis, Cera flava 
and Unguentum leniens are reported from Switzerland. These contact allergies arc probably the result of 
residues of propolis in Cera flava, but Unguentum leniens is also a possible Cera flava containing agent, in 
turn containing propolis.

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